Difference between revisions of "Part:BBa K1362171"
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This part is the C-terminal splicing half in a split sfGFP intein splicing assay. The N-terminal splicing partner is <partinfo>BBa_K1362173</partinfo>, the N- and C-terminal non-splicing control halves are <partinfo>BBa_K1362174</partinfo> and <partinfo>BBa_K1362172</partinfo>, respectively. <partinfo>BBa_K1362170</partinfo> served as positive control. | This part is the C-terminal splicing half in a split sfGFP intein splicing assay. The N-terminal splicing partner is <partinfo>BBa_K1362173</partinfo>, the N- and C-terminal non-splicing control halves are <partinfo>BBa_K1362174</partinfo> and <partinfo>BBa_K1362172</partinfo>, respectively. <partinfo>BBa_K1362170</partinfo> served as positive control. | ||
| − | The assay was used to both characterize the reconstitution of split superfold GFP (<partinfo> | + | The assay was used to both characterize the reconstitution of split superfold GFP (<partinfo>BBa_I746908</partinfo>) as well as to demonstrate the splicing activity of the <i>Npu</i>DnaE split Intein <partinfo>BBa_K1362400</partinfo>. |
Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the [[{{PAGENAME}}:Experience|experience section]] of this part. | Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the [[{{PAGENAME}}:Experience|experience section]] of this part. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 17:16, 10 October 2014
Split sfGFP reconstitution C-terminal splicing half
This part is the C-terminal splicing half in a split sfGFP intein splicing assay. The N-terminal splicing partner is BBa_K1362173, the N- and C-terminal non-splicing control halves are BBa_K1362174 and BBa_K1362172, respectively. BBa_K1362170 served as positive control.
The assay was used to both characterize the reconstitution of split superfold GFP (BBa_I746908) as well as to demonstrate the splicing activity of the NpuDnaE split Intein BBa_K1362400. Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the experience section of this part.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]