Difference between revisions of "Part:BBa K1366102"

Revision as of 01:00, 13 October 2014

Genetic construct for msbB gene deletion (C2)

This construct contains the sequence homologies to delete the msbB gene (Braun’s lipoprotein) with a kanamycin resistance and the sequences for the Lux I production (AHL synthase). The deletion of msbB gene and the replacement of that sequence with the Kan resistance and LuxI will be performed with the red-lambda system. The FTR sequences included are used to remove the antibiotic resistance with a specific recombinase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1108
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 772
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 621
Illegal SapI.rc site found at 831

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1366102 short</partinfo>
-This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.
+This construct contains the sequence homologies to delete the msbB gene (Braun’s lipoprotein) with a kanamycin resistance and the sequences for the Lux I production (AHL synthase). The deletion of msbB gene and the replacement of that sequence with the Kan resistance and LuxI will be performed with the red-lambda system. The FTR sequences included are used to remove the antibiotic resistance with a specific recombinase.
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