Difference between revisions of "Part:BBa K1484101:Design"
 
Line 9: Line 9:
  
 
===Source===
 
===Source===
−
 
+
Originally isolated from S. cerevisiae genome.
−
J. Haseloff
+
Plant optimized CDS: J. Haseloff
  
 
===References===
 
===References===

Latest revision as of 12:00, 10 October 2014

GAL4-VP16 transcription activator CDS, compatible with PlantSyntax (RFC105)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 226
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 695
    Illegal SapI site found at 206


Design Notes

Restriction sites removed for RFC10. Sites added for compatibility with RFC105 (PlantSyntax).

Source

Originally isolated from S. cerevisiae genome. Plant optimized CDS: J. Haseloff

References