Difference between revisions of "Part:BBa K1448000"

Revision as of 07:11, 10 October 2014

gamma-terpinene synthase

gamma-terpinene synthase produces gamma-terpinene from GPP,geranyl diphosphate.

This part contains gamma-terpinene synthase region of Citrus unshiu and was isolated by Mr.Shimada from Citrus unshiu.http://www.sciencedirect.com/science/article/pii/S0168945203003777 Shimada et al, 2003

Background and prnciples

Characterization

Gel picture of our designned part

Westernbloting analysis

(Fig.2 Western-bloting analysis of GST and d-limonene synthase (Lane2), ɤ-terpinene synthase (Lane3) and β-pinene synthase(Lane4) fusion proteins expressed in yeast. (A) The gel picture of SDS-PAGE and western-blot; Lanes: 1, p427-TEF; 2, p427-TEF-LS; 3, p427-TEF-ɤTPNS; 4, p427-TEF-βPINS. (B) vector map of plasmids. (C) The names and molecular weights of each mono-terpene synthase.)

In vivo detection of ɤ-terpinene using GC-MS

Reference

Sequence and Features