Difference between revisions of "Part:BBa K1497019"
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__NOTOC__
 
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<partinfo>BBa_K1497019 short</partinfo>
 
<partinfo>BBa_K1497019 short</partinfo>
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FdeR is a homo dimeric protein from ''Herbaspirillum seropedicae''. In present of naringenin, FdeR activates the specific promotor region upstream of the fdeR region and allow a strong gene expression.  
 
FdeR is a homo dimeric protein from ''Herbaspirillum seropedicae''. In present of naringenin, FdeR activates the specific promotor region upstream of the fdeR region and allow a strong gene expression.  
 
    
 
    
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We modified the naringenin biosensor from Siedler et al. by elimination of the internal EcoRI restriction site and replaced the CFP by the Biobrick Suffix. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin.  
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Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin.  
  
 
[[File:Homology_model_of_FdeR.png|300px|thumb|right|Homology model of FdeR. PDB: 2ESN was used as template. ]]
 
[[File:Homology_model_of_FdeR.png|300px|thumb|right|Homology model of FdeR. PDB: 2ESN was used as template. ]]
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===Usage and Biology==
 
===Usage and Biology==
  
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You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
 
  
  
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<BLOCKQUOTE>
 
<BLOCKQUOTE>
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     <li>GFP K1497020</li>
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     <li>with GFP response      <a href="/Part:BBa_K1497020">K1497020</a></li>
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     <li>mKate K1497021</li>
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     <li>with mKate response  <a href="/Part:BBa_K1497021">K1497021</a></li>
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     <li>CFP K1497022</li>
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     <li>with CFP response <a href="/Part:BBa_K1497022">K1497022</a></li>
 
</BLOCKQUOTE>
 
</BLOCKQUOTE>
 
</html>
 
</html>
  
  
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You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
  
  
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===Functional Parameters===
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:03, 9 October 2014

fdeR (Naringenin binding protein) and fde operon regulatory domain

FdeR is a homo dimeric protein from Herbaspirillum seropedicae. In present of naringenin, FdeR activates the specific promotor region upstream of the fdeR region and allow a strong gene expression.

Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin.

Homology model of FdeR. PDB: 2ESN was used as template.


=Usage and Biology

You can use the reporters for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors:


  • with GFP response K1497020
  • with mKate response K1497021
  • with CFP response K1497022


    • You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.


    Functional Parameters

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 95
      Illegal NgoMIV site found at 452
      Illegal NgoMIV site found at 543
      Illegal NgoMIV site found at 555
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal BsaI.rc site found at 271