Difference between revisions of "Part:BBa K1328002"
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+ | HXT1-CMVmini-EGFP-tADH1 | ||
+ | https://static.igem.org/mediawiki/2014/c/c7/2014-HXT1%2BEGFP%2BADH1.png | ||
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+ | HXT-1+EGFP+ADH1 consists of HXT-1+CMV promoter, EGFP and terminator ADH1. | ||
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+ | HXT-1+CMV promoter controls EGFP expression under the regulation of glucose. | ||
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+ | EGFP is used here for testing of HXT1+CMV promoter and will be replaced by insulin gene. | ||
__NOTOC__ | __NOTOC__ |
Revision as of 15:56, 17 October 2014
HXT1-CMVmini-EGFP-tADH1
HXT-1+EGFP+ADH1 consists of HXT-1+CMV promoter, EGFP and terminator ADH1.
HXT-1+CMV promoter controls EGFP expression under the regulation of glucose.
EGFP is used here for testing of HXT1+CMV promoter and will be replaced by insulin gene.
HXT1-CMVmini-EGFP-tADH1
This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator.
S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 818
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 827
- 1000COMPATIBLE WITH RFC[1000]