Difference between revisions of "Part:BBa K1321116:Design"
(→Source) |
|||
| Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | + | Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part was made by inverse PCR of BBa_J04500, and ligated. | |
| − | + | ||
===References=== | ===References=== | ||
Revision as of 20:19, 24 October 2014
Linker-cipA-linker + phytochelatin (PC) EC20 with LacI promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 383
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 383
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 383
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 383
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 383
Illegal NgoMIV site found at 230 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
CBD10+PhytoC with LacI
Source
Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part was made by inverse PCR of BBa_J04500, and ligated.