Difference between revisions of "Part:BBa K1321110:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Phytochelatin EC(20) +CBD2 with LacI
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Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.
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+
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+
  
 
===Source===
 
===Source===

Revision as of 20:03, 21 October 2014


Phytochelatin (PC) EC20 fused to linker-dCBD with LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.

Source

Phytochelatin EC(20) +CBD2 with LacI

References