Difference between revisions of "Part:BBa K1349002:Design"
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Latest revision as of 07:30, 9 October 2014
CusR box
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
CusR is a response regulator that binds to DNA when phosphorylated. This small part is the recognition squence for the protein and we designed it to be inserted downstream of a promoter to allow negative regulation of transcription by CusR phosphorylation. In common with many response regulators we expect over-expression of CusR to result in binding and so negative regulation of an upstream promoter. These negative effects are expected through bound CusR physically blocking RNA polymerase. This part is complementary to part BBa_K1349003.
A construct with this part between a promoter and a gene is expected to show negative regulation of the gene by phosphorylation of CusR (or overexpression of CusR).
Source
This part was obtained from synthetic oligonucleotide synthesis.