Difference between revisions of "Part:BBa K1455002:Experience"

Revision as of 03:36, 9 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1455002

This device is used to express LuxO D47E by arabinose induction and at the same time to measure the expression of LuxOD 47E in an indirectly way by GFP fluoresence.

LuxOD 47E acts like a phosphorilated LuxO, which is an essential protein of Vibrio spp. quorum sensing system. LuxO is the final protein in a phophorilation cascade and binds, as a trancription facotr, to pqrr promoters. LuxOD 47E is able to activate the genes downstream of a pqrr promoter.

Arabinose inducible promoter used here is a strong one.

The following pictures were taken using a Eclipse-Ti Nikon® ECLIPSE Ti inverted microscope equiped with an ANDOR iXon Ultra 897 camera. The filter used was FITC.

Here is E. coli DH5α carrying this device after one hour of induction with 0.1 % arabinose, light (left) and FITC (right):

And after 3 hours (both with FITC):

User Reviews

UNIQ5a0bbba50666110e-partinfo-00000000-QINU UNIQ5a0bbba50666110e-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K1455002===
+This device is used to express LuxO D47E by arabinose induction and at the same time to measure the expression of LuxOD 47E in an indirectly way by GFP fluoresence.
+LuxOD 47E acts like a phosphorilated LuxO, which is an essential protein of Vibrio spp. quorum sensing system. LuxO is the final protein in a phophorilation cascade and binds, as a trancription facotr, to pqrr promoters. LuxOD 47E is able to activate the genes downstream of a pqrr promoter.
+Arabinose inducible promoter used here is a strong one.
 The following pictures were taken using a Eclipse-Ti Nikon® ECLIPSE Ti inverted microscope equiped with an ANDOR iXon Ultra 897 camera. The filter used was FITC.