Difference between revisions of "Part:BBa K1470006"
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<partinfo>BBa_K1470006 short</partinfo> | <partinfo>BBa_K1470006 short</partinfo> | ||
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Template pMIG | Template pMIG | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <p> pMIG is a novel and easy to handle mammalian expression vector and to create fast and efficiently stable cell lines. It contains Long Terminal Repeats (LTR) for production of viral vectors. There is no packaging signal or gag, pol, env in pMIG. Thus it can be used in any S1 laboratory. | ||
− | < | + | </p> |
+ | <p> | ||
+ | Because of pMIGs retroviral origins, it can be used only in packaging cell lines such as Phoenix cells, to produce viral vectors. Otherwise there won’t be any functional vectors due to the lack of gag, pol, env and the psi-packaging signal. | ||
+ | </p> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
+ | <p>There is a multiple cloning site between the LTRs including EcoRI,PstI,BamHI and many more so you can insert any gene of interest. We suggest easy and reasonable digestion and ligation of an EcoRI- and PstI-site free plasmid backbone. | ||
+ | </p> | ||
<partinfo>BBa_K1470006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1470006 SequenceAndFeatures</partinfo> | ||
Revision as of 20:03, 16 October 2014
pMIG
Template pMIG
Usage and Biology
pMIG is a novel and easy to handle mammalian expression vector and to create fast and efficiently stable cell lines. It contains Long Terminal Repeats (LTR) for production of viral vectors. There is no packaging signal or gag, pol, env in pMIG. Thus it can be used in any S1 laboratory.
Because of pMIGs retroviral origins, it can be used only in packaging cell lines such as Phoenix cells, to produce viral vectors. Otherwise there won’t be any functional vectors due to the lack of gag, pol, env and the psi-packaging signal.
Sequence and Features
There is a multiple cloning site between the LTRs including EcoRI,PstI,BamHI and many more so you can insert any gene of interest. We suggest easy and reasonable digestion and ligation of an EcoRI- and PstI-site free plasmid backbone.
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4917
Illegal XbaI site found at 289
Illegal XbaI site found at 3711
Illegal PstI site found at 10 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4917
Illegal NheI site found at 99
Illegal NheI site found at 3521
Illegal PstI site found at 10
Illegal NotI site found at 4925 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4917
Illegal BglII site found at 4899
Illegal XhoI site found at 4905 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4917
Illegal XbaI site found at 289
Illegal XbaI site found at 3711
Illegal PstI site found at 10 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4917
Illegal XbaI site found at 289
Illegal XbaI site found at 3711
Illegal PstI site found at 10
Illegal NgoMIV site found at 3466
Illegal NgoMIV site found at 4123 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 520
Illegal BsaI site found at 541
Illegal BsaI site found at 3964
Illegal BsaI.rc site found at 1901
Illegal BsaI.rc site found at 4030
Illegal BsaI.rc site found at 4645
Illegal SapI site found at 818