Difference between revisions of "Part:BBa K563004:Experience"
(→User Reviews) |
(→User Reviews) |
||
| Line 37: | Line 37: | ||
The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275. | The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275. | ||
| − | pTEF1 is also listed as parts BBa_K431008 and | + | pTEF1 is also listed as parts BBa_K431008 and BBa_K319003. |
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
Revision as of 18:25, 8 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K563004
User Reviews
UNIQd6b4d19c7d94367c-partinfo-00000000-QINU UNIQd6b4d19c7d94367c-partinfo-00000001-QINU
UNIQd6b4d19c7d94367c-partinfo-00000002-QINU
|
••••
UCSF-UCB iGEM 2014 |
We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter. We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter.
The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275. pTEF1 is also listed as parts BBa_K431008 and BBa_K319003. |
