Difference between revisions of "Part:BBa K1442111"
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<partinfo>BBa_K1442111 short</partinfo> | <partinfo>BBa_K1442111 short</partinfo> | ||
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− | + | == DPP-IV == | |
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+ | As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113). | ||
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− | + | [[File:DPPIV.jpg]] | |
− | + | ''The schematic above shows the organisation of the DPP-IV target'' | |
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+ | == Usage == | ||
− | + | This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing. | |
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Revision as of 15:48, 12 October 2014
DPP-IV (our siRNA target)
DPP-IV
As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113).
The schematic above shows the organisation of the DPP-IV target
Usage
This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.