Difference between revisions of "Part:BBa K1321091:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
PhytoC+CBD4. TBC
+
Codon optimised for E.coli.  
−
 
+
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.
−
 
+
  
 
===Source===
 
===Source===

Revision as of 17:47, 21 October 2014


Phytochelatin (PC) EC20 fused to CBDclos


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.

Source

PhytoC+CBD4. TBC

References