Difference between revisions of "Part:BBa K1548001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1548001 short</partinfo> | <partinfo>BBa_K1548001 short</partinfo> | ||
− | + | Hsp60 promoter (M. bovis) | |
+ | Strong constitutive promoter from ''M. bovis'' and fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium. | ||
+ | References: 1. Oldfield LM, Hatfull GF. Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J Bacteriol. 2014 Oct 15;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4. | ||
+ | Sequence and Features | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 01:24, 18 October 2014
Hsp60 promoter (M. bovis) and RBS (mycobacteriophage TM4)
Hsp60 promoter (M. bovis) Strong constitutive promoter from M. bovis and fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium.
References: 1. Oldfield LM, Hatfull GF. Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J Bacteriol. 2014 Oct 15;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4. Sequence and Features Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 202
- 1000COMPATIBLE WITH RFC[1000]