Difference between revisions of "Part:BBa K1371040"

 
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== Standardization of Interchangeable Domains ==
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The standardization of sufficient numbers of domains from numerous organisms would allow customized replacement, rearrangement, and/or recombining of different domains to produce novel polyketides. In this part, we tested customized synthesis of triketide lactones by replacing the loading domain and swapping KR+ACP domains between modules to test the compatibility of the standardized domains.
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<div class="center">
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[[File:Pict10.png]]
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[[File:Pict12.png]]
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</div>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:30, 24 October 2014

DEBS1(KR1)


Standardization of Interchangeable Domains

The standardization of sufficient numbers of domains from numerous organisms would allow customized replacement, rearrangement, and/or recombining of different domains to produce novel polyketides. In this part, we tested customized synthesis of triketide lactones by replacing the loading domain and swapping KR+ACP domains between modules to test the compatibility of the standardized domains.

Pict10.png Pict12.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1876
    Illegal BamHI site found at 6423
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 2039
    Illegal NgoMIV site found at 2411
    Illegal NgoMIV site found at 2444
    Illegal NgoMIV site found at 2917
    Illegal NgoMIV site found at 3626
    Illegal NgoMIV site found at 4499
    Illegal AgeI site found at 367
    Illegal AgeI site found at 1729
    Illegal AgeI site found at 2075
    Illegal AgeI site found at 2654
    Illegal AgeI site found at 4375
    Illegal AgeI site found at 4445
    Illegal AgeI site found at 5072
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1481
    Illegal BsaI.rc site found at 3911
    Illegal SapI.rc site found at 2746