Difference between revisions of "Part:BBa K1317003:Experience"

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'''Polymer formation trial:'''
 
'''Polymer formation trial:'''
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[[file:2014-10-07_20_25_40-iGEM_2014_-_Bordeaux.png|300px|thumb|right|Fiber obtained by extruding ELP/Alginate in calcium chloride]]
  
 
In order to obtain polymer wires, we carried out severeal tests by mixing the ELP with different solvants. The following results have been observed:
 
In order to obtain polymer wires, we carried out severeal tests by mixing the ELP with different solvants. The following results have been observed:
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- Heated Sodium Chloride (NaCl 5M) => Formation of a white cloud due to a lack of cohesiveness  
 
- Heated Sodium Chloride (NaCl 5M) => Formation of a white cloud due to a lack of cohesiveness  
  
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- Calcium Chloride (CaCl2 15%) => 1.25% alginate mixture with ELP40 at 10mg/mL: a white-colored fiber is obtained
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- Calcium Chloride (CaCl2 15%) => 1.25% alginate mixture with ELP40 at 10mg/mL were extruded in the calcium chloride solution: a white-colored fiber is obtained.
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This last trial has been performed with 0.75% alginate which seems to be the best experimental condition to get fibers from the ELP
 
This last trial has been performed with 0.75% alginate which seems to be the best experimental condition to get fibers from the ELP
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'''Phase transition trial:'''
 
'''Phase transition trial:'''
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Thanks to the mixture alginate + ELP40 (10 mg/L), we could observe the formation of the polymer and it can be deduced that:
 
Thanks to the mixture alginate + ELP40 (10 mg/L), we could observe the formation of the polymer and it can be deduced that:
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- In presence of salt, product is losing cohesiveness
 
- In presence of salt, product is losing cohesiveness
  
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'''Wet-spinning and mechanical testing of fibers properties'''
 
'''Wet-spinning and mechanical testing of fibers properties'''
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The wet-spinning method has been used to create a wire out of the polymer.
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<center>[[file:TeamBdx2014_Wet_spin.gif]]</center>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 19:34, 7 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1317003

The protein ELP has been produced in BL21 strain of E.coli. After phase cycling purification it is possible to spin the protein into a wire, thanks to the wet-spinning method. The ELP can have different length regarding the number of copy of the gene. In these experiments, we used 40mer ELP (assemble of two gene copies).

Polymer formation trial:

Fiber obtained by extruding ELP/Alginate in calcium chloride

In order to obtain polymer wires, we carried out severeal tests by mixing the ELP with different solvants. The following results have been observed:

- Sodium Sulfate (Na2So4) at 320 g/L => cottony aspect of the fiber, the structure is not stable

- Diethyl ether (C2H5)2O => No reaction because the drop is trapped. This solvent is not miscible with the polymer

- Acetone (CH3COCH3) => Formation of a white ring

- Heated Sodium Chloride (NaCl 5M) => Formation of a white cloud due to a lack of cohesiveness

- Calcium Chloride (CaCl2 15%) => 1.25% alginate mixture with ELP40 at 10mg/mL were extruded in the calcium chloride solution: a white-colored fiber is obtained.

This last trial has been performed with 0.75% alginate which seems to be the best experimental condition to get fibers from the ELP


Phase transition trial:

After getting the ELP40 fiber, it is necessary to wash it with hot water for extractio, the fiber could indeed dissolve at low temperature.

- Control remains transparent

- The aspect of the fiber changes at ambient temperature (from white to transprent)

Thanks to the mixture alginate + ELP40 (10 mg/L), we could observe the formation of the polymer and it can be deduced that:

- In presence of salt, product is losing cohesiveness

- With contact of hot water, the product is contracting

You can see the results on the phase transition on this video: https://www.youtube.com/watch?v=xoTQkoNoZu0

Wet-spinning and mechanical testing of fibers properties

The wet-spinning method has been used to create a wire out of the polymer.

TeamBdx2014 Wet spin.gif

User Reviews

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