Difference between revisions of "Part:BBa K1378005"
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This composite part is fused by INP,MVN and mRFP.It can display MVN protein  on the surface of E.coli through INP and emission red fluorecence. We use this part to detect whether MVN is translocated on the out membrane.
 
This composite part is fused by INP,MVN and mRFP.It can display MVN protein  on the surface of E.coli through INP and emission red fluorecence. We use this part to detect whether MVN is translocated on the out membrane.
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''we''
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<experiments>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:29, 7 October 2014

INPNC-linker2-MVN-linker1-mRFP

This composite part is fused by INP,MVN and mRFP.It can display MVN protein on the surface of E.coli through INP and emission red fluorecence. We use this part to detect whether MVN is translocated on the out membrane.

<experiments> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1915
    Illegal AgeI site found at 2027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1029