Difference between revisions of "Part:BBa K1442106"

 
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<partinfo>BBa_K1442106 short</partinfo>
 
<partinfo>BBa_K1442106 short</partinfo>
  
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Forward GFP for use in human cell tests.
 
  
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== FG ==
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===Usage and Biology===
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The forward GFP for human cells is a specially designed testing module. It contains GFP codon optimised to work in human cells. This testing module can be used to test internal ribosome entry sites (IRES).
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[[File:FG.jpg]]
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''This schematic developed in Geneious shows the organisation of the FG testing module''
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1442106 SequenceAndFeatures</partinfo>
 
  
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== Usage ==
  
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The FG was intended to be used to test the EMCV IRES (BBa_K1442023) and the NKRF IRES (BBa_K1442024) using the restriction sites AscI and BsrGI before the GFP. After the GFP are two more restriction sites Mau BI and Mlu I that can be used to test the Aptazyme (BBa_K1442006) and the DPP-IV target (BBa_K1442111).
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===Functional Parameters===
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<partinfo>BBa_K1442106 parameters</partinfo>
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Revision as of 15:06, 12 October 2014

Forward GFP for human cells


FG

The forward GFP for human cells is a specially designed testing module. It contains GFP codon optimised to work in human cells. This testing module can be used to test internal ribosome entry sites (IRES).

FG.jpg

This schematic developed in Geneious shows the organisation of the FG testing module


Usage

The FG was intended to be used to test the EMCV IRES (BBa_K1442023) and the NKRF IRES (BBa_K1442024) using the restriction sites AscI and BsrGI before the GFP. After the GFP are two more restriction sites Mau BI and Mlu I that can be used to test the Aptazyme (BBa_K1442006) and the DPP-IV target (BBa_K1442111).