Difference between revisions of "Part:BBa K1526008"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1526008 short</partinfo> | <partinfo>BBa_K1526008 short</partinfo> | ||
− | + | Lac Repressor System + CysP | |
− | + | The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family | |
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+ | <b>Gene:</b> cysP (AKA YlnA) <br> | ||
+ | <b>Organism:</b> <i>Bacillus Subtilis</i> <br> | ||
+ | <b>Protein:</b> CysP <br> | ||
+ | <b>Function:</b> This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space. <br> | ||
+ | <b>Aim:</b> We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium. <br> | ||
+ | <b>Expression and characterization:</b> In order to characterise the sulfate secondary transporter from <i>Bacillus subtilis</i> we have acquired <i>E. coli BW25113</i> strain mutants from the Keio collection for complementary assays. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 18:05, 18 October 2014
LacRS + CysP
Lac Repressor System + CysP
The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family
Gene: cysP (AKA YlnA)
Organism: Bacillus Subtilis
Protein: CysP
Function: This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space.
Aim: We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium.
Expression and characterization: In order to characterise the sulfate secondary transporter from Bacillus subtilis we have acquired E. coli BW25113 strain mutants from the Keio collection for complementary assays.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1207
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2102
Illegal NgoMIV site found at 2220 - 1000COMPATIBLE WITH RFC[1000]