Difference between revisions of "Part:BBa K1362421:Design"

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===Design Notes===
 
===Design Notes===
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This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
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This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[105] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
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===Source===
 
===Source===

Revision as of 15:43, 24 October 2014


C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[105] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.

Source

This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [1]. All standard sequences can be reviewed in RFC[???] [2].

References

[1] iGEM team Heidelberg 2014

[2] RFC[???]