Difference between revisions of "Part:BBa K1526007"
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<partinfo>BBa_K1526007 short</partinfo> | <partinfo>BBa_K1526007 short</partinfo> | ||
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− | + | <p>Gene: mntH | |
+ | <p>Organism: Escherichia Coli | ||
+ | <p>Protein: MntH transporter from NRAMP family | ||
+ | <p>Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell. | ||
+ | <p>Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process. | ||
+ | Expression: We can run assays measuring the concentration of cadmium in the environment. If NRAMP is active, the concentration of cadmium should decrease. | ||
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Revision as of 15:07, 18 October 2014
LacRS + MntH
lacI + NRAMP
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1188
Illegal BamHI site found at 2462 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2358
- 1000COMPATIBLE WITH RFC[1000]