Difference between revisions of "Part:BBa K1526003"
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<partinfo>BBa_K1526003 short</partinfo> | <partinfo>BBa_K1526003 short</partinfo> | ||
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The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family | The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family | ||
− | < | + | <b>Gene:</b> cysP (AKA YlnA) <br> |
− | + | <b>Organism:</b> <i>Bacillus Subtilis</i> <br> | |
+ | <b>Protein:</b> CysP <br> | ||
+ | <b>Function:</b> This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space. <br> | ||
+ | <b>Aim:</b> We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium. <br> | ||
+ | <b>Expression:</b> We could run assays measuring the concentration of sulfate in the environment containing our bacteria. | ||
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Revision as of 15:43, 18 October 2014
CysP from Bacillus subtilis
The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family
Gene: cysP (AKA YlnA)
Organism: Bacillus Subtilis
Protein: CysP
Function: This gene encodes for CysP, a sulfate/thiosulfate ABC transporter found in the periplasmic space.
Aim: We want to over-express this gene in E.coli. This would lead to increased sulfate uptake, which could be used to produce cysteine and eventually phytochelatins that would bind cadmium.
Expression: We could run assays measuring the concentration of sulfate in the environment containing our bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 680 - 1000COMPATIBLE WITH RFC[1000]