Difference between revisions of "Part:BBa K1497004"
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+ | The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited. | ||
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+ | src="https://static.igem.org/mediawiki/parts/1/1c/CHS_Reaktionsgleichung.png"></p> | ||
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+ | <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US"> | ||
+ | Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.</span></p> | ||
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For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034. | For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034. | ||
Revision as of 11:13, 11 October 2014
B0034-CHS - Chalcone Synthase from Gerbera with strong RBS
The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in E. coli the source of Malonyl-CoA is limited. |
Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone. |
For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 543
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 552
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 523
Illegal NgoMIV site found at 1099 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 472