Difference between revisions of "Part:BBa F2620"

Revision as of 15:58, 5 October 2006

Input:	3OC₆HSL
Output:	PoPS

tetR R0040	LuxR I0462	lux pR R0062

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing:	0.1 - 1000 nM 3OC₆HSL, exogenous	L/H Response time:	25 mins
Output Swing:	13.6 - 490.2 GFP molecules/s.cfu	Genetic Stability:	>92/74 generations (Low/High Input)
Switch Point:	8 nM 3OC₆HSL, exogenous	Performance Stability:	>92/74 generations (Low/High Input)

Transfer Function	Specificity
Response time	Stability

Demand
Transcription Demand:	## NTP per second ## polymerases sequestered	Translation Demand:	# charged tRNA per second # ribosomes sequestered

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with devices using 3OC₆HSL or add in the other molecule names here. Crosstalk with systems containing BBa_C0040.

@@ Line 17: / Line 17: @@
 |13.6 - 490.2 GFP molecules/s.cfu
 |align='left'|[[Genetic Stability|''Genetic Stability:'']]
-|92/74 generations (Low/High Input)
+|>92/74 generations (Low/High Input)
 |-
 |[[Switch Point|''Switch Point:'']]
-|6 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
+|8 nM [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
 |align='left'|[[Performance Stability|''Performance Stability:'']]
-|92/74 generations (Low/High Input)
+|>92/74 generations (Low/High Input)
 |}
 {|align="center" cellspacing="0" cellpadding="0"