Difference between revisions of "Part:BBa K1379004:Design"
(→‎Source)
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===Source===
 
===Source===
−
- sigmaX gene awas cloned from genomic DNA of Streptococcus pneumoniae NCTC7465 strain<br>
+
iGEM 2014 Hong_Kong_HKUST Team has cloned &sigma;<sup>X</sup> from <i>S. pneumoniae</i> strain NCTC7465 by PCR, followed by restriction-ligation into [[Part:pSB1C3|pSB1C3]] backbone.
  
 
===References===
 
===References===

Revision as of 15:06, 8 October 2014

S. pneumoniae σx CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 298


Design Notes

None

Source

iGEM 2014 Hong_Kong_HKUST Team has cloned σX from S. pneumoniae strain NCTC7465 by PCR, followed by restriction-ligation into pSB1C3 backbone.

References