Difference between revisions of "Part:BBa F2620"

Revision as of 23:42, 3 October 2006

Input:	3OC₆HSL
Output:	PoPS

tetR R0040	LuxR I0462	lux pR R0062

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics

Input Swing:	0.1 - 1000 nM 3OC₆HSL, exogenous
Output Swing:	13.6 - 490.2 GFP molecules/s.cfu
Switch Point:	6 nM 3OC₆HSL, exogenous
L/H Response time:	25 mins

Key Subparts

BBa_R0040:	TetR-regulated operator
BBa_C0062:	luxR ORF
BBa_R0062:	LuxR-regulated operator

Transfer Function	Specificity
Response time	Stability

Demand

Transcription Demand:	## NTP per second ## polymerases sequestered
Translation Demand:	# charged tRNA per second # ribosomes sequestered

Stability

Genetic Stability:	92/74 generations (Low/High Input)
Performance Stability:	92/74 generations (Low/High Input)

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmid: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC₆HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part BBa_T9002, ([BBa_F2620].[BBa_E0240]). The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.

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