Difference between revisions of "Part:BBa K1400004:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1400004=== | ===Applications of BBa_K1400004=== | ||
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| + | == uOttawa 2015 == | ||
| + | The original application for this promoter was to use it to implement a tri-stable switch, which is thought to be involved in stem cell differentiation. However, when we were analyzing our models more in-depth, we found that the dynamics of this promoter do not lead to tri-stability. | ||
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| + | Specifically, this promoter behaves ''multiplicatively''. This means that adding a fixed amount of repressor will down-regulate the gene based on how much it is induced. For instance, adding a certain amount of repressor will cut the promoter's expression in half, rather than lowering it by a fixed amount. | ||
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| + | The promoter also seems to behave differently when doxycycline is used instead of aTc to modulate repression by rtTA. With doxycycline, repression seems to be much weaker and activation with beta-estradiol modulating GEV seems to have a greater effect on the activity of the promoter. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 21:29, 18 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1400004
uOttawa 2015
The original application for this promoter was to use it to implement a tri-stable switch, which is thought to be involved in stem cell differentiation. However, when we were analyzing our models more in-depth, we found that the dynamics of this promoter do not lead to tri-stability.
Specifically, this promoter behaves multiplicatively. This means that adding a fixed amount of repressor will down-regulate the gene based on how much it is induced. For instance, adding a certain amount of repressor will cut the promoter's expression in half, rather than lowering it by a fixed amount.
The promoter also seems to behave differently when doxycycline is used instead of aTc to modulate repression by rtTA. With doxycycline, repression seems to be much weaker and activation with beta-estradiol modulating GEV seems to have a greater effect on the activity of the promoter.
User Reviews
UNIQ3e8f422ac787a86a-partinfo-00000000-QINU UNIQ3e8f422ac787a86a-partinfo-00000001-QINU