Difference between revisions of "Part:BBa J23101"

Revision as of 18:39, 31 October 2006

constitutive promoter family member

 Variant RFP (au)
 J23112           1
 J23103           17
 J23113           21
 J23109           106
 J23117           162
 J23114           256
 J23115           387
 J23116           396
 J23105           623
 J23110           844
 J23107           908
 J23106           1185
 J23108           1303
 J23118           1429
 J23111           1487
 J23101           1791
 J23104           1831
 J23102           2179
 J23100           2547

Constitutive promoter family
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescence of these plasmids in strain TG1 grown in LB media to saturation. See part BBa_J61002 for details on their use.

These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for further modification. JCAraw

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J23101 short</partinfo>
+<!--[[Template:Berk2006iGEM_JCA_TCPromoters]]-->
+{{Berk2006iGEM_JCA_TCPromoters}}
-[[Image:BerkiGEM2006-Promoters.jpg|300px|left]]
-[[Image:BerkiGEM2006-PromotersEppendorfs.jpg|300px]]<br>
-<tt>
-Variant RFP (au)
-J23112 1   <br>
-J23103 17  J23107 908<br>
-J23113 21  J23106 1185<br>
-J23109 106 J23108 1303<br>
-J23117 162 J23118 1429<br>
-J23114 256 J23111 1487<br>
-J23115 387 J23101 1791<br>
-J23116 396 J23104 1831<br>
-J23105 623 J23102 2179<br>
-J23110 844 J23100 2547<br>
-<br>
-</tt>
-----
-[[Image:pBca1020-r0040.jpg|left|180px]]<br>
-'''Constitutive promoter family'''<br>
-Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library.  J23119 is the "consensus" promoter sequence and the strongest member of the family.  All parts except J23119 are present in plasmid J61002.  Part J23119 is present in pSB1A2.  This places the RFP downstream of the promoter.  Reported activities of the promoters are given as the relative fluorescense of these plasmids in strain TG1 grown in LB media to saturation.  See part J61002 for details on their use.
-These promoter parts can be used to tune the expression level of constitutively expressed parts.  The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for furhter modification.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===