Difference between revisions of "Part:BBa K1362411:Design"
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===References=== | ===References=== | ||
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+ | [1] https://www.addgene.org/11962/ | ||
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+ | [2] Sara Züger & Hideo Iwai Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies Nature Biotechnology 23, 736 - 740 (2005) | ||
+ | Published online: 22 May 2005 | doi:10.1038/nbt1097" |
Revision as of 18:52, 6 October 2014
SspDnaE C-Intein cloning piece
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.
Source
Part sequence and DNA were obtained from Addgene (pSFBAD09) [1]. Further Information came amongst other literature from Iwai et al. [2]
References
[1] https://www.addgene.org/11962/
[2] Sara Züger & Hideo Iwai Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies Nature Biotechnology 23, 736 - 740 (2005) Published online: 22 May 2005 | doi:10.1038/nbt1097"