Difference between revisions of "Part:BBa F2620"
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|Last Update=15th Sept. 2006
 
|Last Update=15th Sept. 2006
 
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'''Description'''<br>
 
'''Description'''<br>
 
A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
 
A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
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|width='340px'|'''Characteristics'''
 
|width='340px'|'''Characteristics'''
 
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|width='245px'|0.1 - 1000 nM 3OC<sub>6</sub>HSL, exogenous
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|width='210px'|0.1 - 1000 nM 3OC<sub>6</sub>HSL, exogenous
 
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|[[Output Swing|''Output Swing:'']]
 
|[[Output Swing|''Output Swing:'']]
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|6 nM 3OC<sub>6</sub>HSL, exogenous
 
|6 nM 3OC<sub>6</sub>HSL, exogenous
 
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|[[Latency|''L/H Latency:'']]
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|25 mins
 
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|LuxR-regulated operator<br>
 
|LuxR-regulated operator<br>
 
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|width='375px' align="left"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]<br>
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{{Click || image=BC-TransferCurve-3.png | link=Part:BBa_F2620:Transfer Function |width=300px | height=200px }}
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|width='375px' align="right"|[[Part:BBa_F2620:Specificity|'''Specificity''']]<br>
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{{Click || image=BC-Specificity-1.png | link=Part:BBa_F2620:Specificity |width=300px | height=200px }}
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|align="left"|[[Part:BBa_F2620:Response time|'''Response time''']]<br>
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{{Click || image=BC-Latency-2.png | link=Part:BBa_F2620:Response time |width=300px | height=200px }}
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|align="right"|[[Part:BBa_F2620:Stability|'''Stability''']]<br>
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{{Click || image=BC-Stability-1.png | link=Part:BBa_F2620:Stability |width=300px | height=250px }}
 
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Revision as of 22:36, 3 October 2006

Input: 3OC6HSL
Output: PoPS

tetR
R0040		LuxR
I0462		lux pR
R0062
 BBa F2620Icon.png

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing: 0.1 - 1000 nM 3OC6HSL, exogenous
Output Swing: 13.6 - 490.2 GFP molecules/s.cfu
Switch Point: 6 nM 3OC6HSL, exogenous
L/H Response time: 25 mins
Key Subparts
BBa_R0040: TetR-regulated operator
BBa_C0062: luxR ORF
BBa_R0062: LuxR-regulated operator
Transfer Function
 Specificity
Response time
 Stability
Demand
Transcription Demand: ## NTP per second
## polymerases sequestered
Translation Demand: # charged tRNA per second
# ribosomes sequestered
Stability
Genetic Stability: 92/74 generations (Low/High Input)
Performance Stability: 92/74 generations (Low/High Input)

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmid: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part BBa_T9002, ([BBa_F2620].[BBa_E0240]). The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.

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