Difference between revisions of "Part:BBa K1351024"
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<partinfo>BBa_K1351024 short</partinfo>
 
<partinfo>BBa_K1351024 short</partinfo>
  
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CSP-depending promoter of ''Streptococcus pneumoniae''.
+
CSP-depending promoter of ''Streptococcus pneumoniae''(R6).
  
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This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:  
+
This part was generated with the RFC10 standard and has the following prefix and suffix:  
  
 
{|
 
{|
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|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:  
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|prefix with EcoRI, NotI and XbaI:  
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|<span style="color:blue">GAATTCC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>T<u>AAGGAGG</u>A<span style="color:orange">GCCGGC</span>
+
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>G
 
|-
 
|-
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|suffix with AgeI, SpeI, NotI and PstI:  
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|suffix with SpeI, NotI and PstI:  
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|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>
+
|T<span style="color:red">ACTAGT</span>A<span style="color:green">GCGGCCG</span><span style="color:blue">CTGCAG</span>
 
|}
 
|}
 
Sites of restriction enzymes generating compatible overhangs have the same color:
 
Sites of restriction enzymes generating compatible overhangs have the same color:
  
−
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Shine-Dalgarno sequence and stop codons are underlined.
+
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red.
−
 
+
  
  

Revision as of 07:18, 7 October 2014

PcomC, a CSP inducible promoter derived from Streptococcus pneumoniae

CSP-depending promoter of Streptococcus pneumoniae(R6).

This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI: TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]