Difference between revisions of "Part:BBa K1510011:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1510011 short</partinfo> | <partinfo>BBa_K1510011 short</partinfo> | ||
| Line 14: | Line 13: | ||
J23100 is a strong constitutive promoter from 2014 igem distribution. The coding region, yebF and endolysin come from different origin. YebF is from E.coli K12, while endolysin is from the 19th opening reading frame of Streptococcus Mutans phage M102. Finally, B0015 is a double terminator from igem distribution. | J23100 is a strong constitutive promoter from 2014 igem distribution. The coding region, yebF and endolysin come from different origin. YebF is from E.coli K12, while endolysin is from the 19th opening reading frame of Streptococcus Mutans phage M102. Finally, B0015 is a double terminator from igem distribution. | ||
| + | <p> | ||
| + | [[File:NYMU chienyunghuang endolysin KODJPEG.jpg]] | ||
| + | </p> | ||
===References=== | ===References=== | ||
Revision as of 19:03, 17 October 2014
Extracellular endolysin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 159
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The endolysin sequence from phage M102 contains a Spe1 cutting site, therefore, we design primers to make point mutation of the sequence in order to produce the same amino acid without the risk of being cut during circuit construction.
Source
J23100 is a strong constitutive promoter from 2014 igem distribution. The coding region, yebF and endolysin come from different origin. YebF is from E.coli K12, while endolysin is from the 19th opening reading frame of Streptococcus Mutans phage M102. Finally, B0015 is a double terminator from igem distribution.
