Difference between revisions of "Part:BBa K1351028"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1351028 short</partinfo>
 
<partinfo>BBa_K1351028 short</partinfo>
  
This part was generated in a modified version of RFC25, and has the following prefix and suffix:  
+
This part was generated in a modified version of RFC10, and has the following prefix and suffix:  
  
 
{||prefix with EcoRI, NotI, XbaI, SD and NgoMIV:|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>|-|suffix with AgeI, SpeI, NotI and PstI:|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>|}Sites of restriction enzymes generating compatible overhangs have the same color:
 
{||prefix with EcoRI, NotI, XbaI, SD and NgoMIV:|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>|-|suffix with AgeI, SpeI, NotI and PstI:|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>|}Sites of restriction enzymes generating compatible overhangs have the same color:

Revision as of 11:26, 17 October 2014

LMU Bacillus RBS collection 1

This part was generated in a modified version of RFC10, and has the following prefix and suffix:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]