Difference between revisions of "Part:BBa K1314000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | PCR with 30bp primers. One EcoRI site is removed by overlapping PCR. | |
Latest revision as of 04:16, 2 November 2014
Ammonium repressible nifH promoter(from Azotobacter vinelandii)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR with 30bp primers. One EcoRI site is removed by overlapping PCR.
Source
A. vinelandii(strain DJ)