Difference between revisions of "Part:BBa K1354000"

Latest revision as of 17:19, 5 October 2014

UV Promoter with Phage Activator

This is a composite part that used as the input component for our 2-plasmid system. The input BioBrick consists of a promoter gene, in this case it is a SulA UV promoter, with an RBS, the PhiR73 Phage promoter, and a double Terminator. The composite part is flanked by an XhoI site and a EcoRI site. Between the UV promoter and the Phage activator there is a KpnI site that can be used as a way to cut the UV promoter out to swap in other promoters. Before the UV promoter there is a BioBrick prefix. There are start and stop codons flanking the Phage Promoter, along with a scar site. After the double Terminator there is a BioBrick suffix.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 17:18, 5 October 2014 (view source) Devoranajjar (Talk \| contribs) ← Older edit		Latest revision as of 17:19, 5 October 2014 (view source) Devoranajjar (Talk \| contribs)
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	<partinfo>BBa_K1354000 short</partinfo>		<partinfo>BBa_K1354000 short</partinfo>

−	This is a composite part that used as the input component for our 2-plasmid system. The input BioBrick consists of a promoter gene, in this case it is a SulA UV promoter, with an RBS ~~and~~ the PhiR73 Phage promoter. The composite part is flanked by an XhoI site and a EcoRI site. Between the UV promoter and the Phage activator there is a KpnI site that can be used as a way to cut the UV promoter out to swap in other promoters. Before the UV promoter there is a BioBrick prefix. There are start and stop codons flanking the Phage Promoter, along with a scar site. After the double Terminator there is a BioBrick suffix.	+	This is a composite part that used as the input component for our 2-plasmid system. The input BioBrick consists of a promoter gene, in this case it is a SulA UV promoter, with an RBS, the PhiR73 Phage promoter, and a double Terminator. The composite part is flanked by an XhoI site and a EcoRI site. Between the UV promoter and the Phage activator there is a KpnI site that can be used as a way to cut the UV promoter out to swap in other promoters. Before the UV promoter there is a BioBrick prefix. There are start and stop codons flanking the Phage Promoter, along with a scar site. After the double Terminator there is a BioBrick suffix.