Difference between revisions of "Part:BBa K1475005:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1475005 short</partinfo>
 
<partinfo>BBa_K1475005 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part was constructed using standard assembly from the iGEM registry parts [https://parts.igem.org/Part:BBa_K1475004 Part:BBa_K1475004] and  [https://parts.igem.org/Part:BBa_E0840 Part:BBa_E0840].
  
  
  
 
===Source===
 
===Source===
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TetR: ”Escherichia coli”
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<br>
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GFP: ”Aequeora victoria” (GFPmut3b) [3]
  
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===References===
 
===References===
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1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
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<br>
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2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
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<br>
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3. Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). http://www.sciencedirect.com/science/article/pii/0378111995006850

Revision as of 12:30, 16 October 2014

GFP controlled by TetR and pTet.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1390


Design Notes

This part was constructed using standard assembly from the iGEM registry parts Part:BBa_K1475004 and Part:BBa_E0840.


Source

TetR: ”Escherichia coli”
GFP: ”Aequeora victoria” (GFPmut3b) [3]


References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
3. Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). http://www.sciencedirect.com/science/article/pii/0378111995006850