Difference between revisions of "Part:BBa K1537024"
| Line 4: | Line 4: | ||
The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- | The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- | ||
hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco. | hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco. | ||
| + | |||
[[File:hps1.jpg]] | [[File:hps1.jpg]] | ||
| + | |||
Fig.1 Schematic Representation of the Bacterial RuMP Pathway and the Plant Calvin-Benson Cycle. | Fig.1 Schematic Representation of the Bacterial RuMP Pathway and the Plant Calvin-Benson Cycle. | ||
HPS and PHI denote 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase respectively. The abbreviations for several sugar phosphates are as follows: Ru5P, ribulose 5-phosphate; Hu6P, hexulose 6-phosphate; F6P, fructose 6-phosphate; FBP, fructose 1,6-bisphosphate;RuBP,ribulose1,5-bisphosphate;3-PGA,3-phosphoglyce-rate. The other metabolites in the pathway are symbolized merely by their carbon numbers for simplicity. | HPS and PHI denote 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase respectively. The abbreviations for several sugar phosphates are as follows: Ru5P, ribulose 5-phosphate; Hu6P, hexulose 6-phosphate; F6P, fructose 6-phosphate; FBP, fructose 1,6-bisphosphate;RuBP,ribulose1,5-bisphosphate;3-PGA,3-phosphoglyce-rate. The other metabolites in the pathway are symbolized merely by their carbon numbers for simplicity. | ||
| + | |||
[[File:hps2.jpg]] | [[File:hps2.jpg]] | ||
| + | |||
Fig.2 AB1 and AB2, two lines transformed with both rmpA(the gene for HPS) and rmpB(the gene for PHI); CK, a negativecontrol line transformed with the empty vector. | Fig.2 AB1 and AB2, two lines transformed with both rmpA(the gene for HPS) and rmpB(the gene for PHI); CK, a negativecontrol line transformed with the empty vector. | ||
Revision as of 14:58, 5 October 2014
HPS-PHI
The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco.
Fig.1 Schematic Representation of the Bacterial RuMP Pathway and the Plant Calvin-Benson Cycle. HPS and PHI denote 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase respectively. The abbreviations for several sugar phosphates are as follows: Ru5P, ribulose 5-phosphate; Hu6P, hexulose 6-phosphate; F6P, fructose 6-phosphate; FBP, fructose 1,6-bisphosphate;RuBP,ribulose1,5-bisphosphate;3-PGA,3-phosphoglyce-rate. The other metabolites in the pathway are symbolized merely by their carbon numbers for simplicity.
Fig.2 AB1 and AB2, two lines transformed with both rmpA(the gene for HPS) and rmpB(the gene for PHI); CK, a negativecontrol line transformed with the empty vector.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1125