Difference between revisions of "Part:BBa J61002"

Revision as of 22:19, 21 September 2006

EX-Ptet-S-rbsRFP-P "RFP reporter"

Plasmid for construction of promoter basic parts and their derivatives. This plasmid can be used two ways:

Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
Insertion of a protein generating device or RNA gene (cutting the part with XbaI/PstI, inserting into SpeI/PstI of J61002) results in a standard pSB1A2 plasmid containing an r0040.yourpart composite.

See similar part J61003 for a similar method of construction for constructing open reading frame parts, or r0040.b0034.your ORF composite parts in pSB1A2. The same procedure can be used with part J23019 for the same effect in a pSB3C6 plasmid backbone.

ApE file:{pBca1020-R0040}

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2927
Illegal XbaI site found at 2942
Illegal SpeI site found at 2
Illegal PstI site found at 885
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2927
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal NotI site found at 878
Illegal NotI site found at 2933
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2927
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2927
Illegal XbaI site found at 2942
Illegal SpeI site found at 2
Illegal PstI site found at 885
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2927
Illegal XbaI site found at 2942
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal AgeI site found at 581
Illegal AgeI site found at 693
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1966

@@ Line 3: / Line 3: @@
 [[Image:pBca1020-r0040.jpg|center|300px]]<br>
-Plasmid for construction of promoter basic parts.  Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly.  Part J61002 is the tet promoter variant of the plasmid.
+Plasmid for construction of promoter basic parts and their derivatives.  This plasmid can be used two ways:
+#Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly.  Part J61002 is the tet promoter variant of the plasmid.
+#Insertion of a protein generating device or RNA gene (cutting the part with XbaI/PstI, inserting into SpeI/PstI of J61002) results in a standard pSB1A2 plasmid containing an r0040.yourpart composite.
+See similar part J61003 for a similar method of construction for constructing open reading frame parts, or r0040.b0034.your ORF composite parts in pSB1A2.  The same procedure can be used with part J23019 for the same effect in a pSB3C6 plasmid backbone.
 [[Media:pBca1020-R0040.ogg|ApE file:{pBca1020-R0040}]]