Difference between revisions of "Part:BBa J100176:Experience"

Revision as of 14:30, 2 October 2014

Purpose: We are testing if triclosan induces the FapR promoter in E.coli cells by fatty acid deprivation.

Method: We collected four E.coli colonies from the experimental plate, two green and two red. We also collected two green colonies from the positive control plate. We mixed these abstracted colonies with LB broth and ampicillin. We chose three of the six tubes prepared and added 20 µL of 0.5% triclosan from antimicrobial hand soap to one experimental, one negative, and one positive tube. The other three tubes do not have triclosan. We used the Synergy machine to measure how much RFP was in each of the six tubes. We also measured the RFP of a blank well, which contained nothing but LBM media. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells. Then we generate the graph using the ratio of red fluorescence protein to cell density.

Result:

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-[[File:Printergraph1.jpg]]
-This figure shows FapR induction in  E. coli by Bacdown hand soap, which contains 0.5% triclosan and surfactants. Because the soap contains surfactants, the bacteria died. The promoter was still able to minimally bind to the cell without the triclosan. We were able to measure the fluorescence of our data by subtracting the fluorescence and absorbance of LBM media from the fluorescence and absorbance of the triclosan treated and non-treated cells.<br>
 [[File:Printergraph1.jpg]]