Difference between revisions of "Part:BBa K1453000"

Revision as of 12:39, 16 October 2014

ssDsbA-mRFP-Lgt-TAL USB-His Tag

This part is designed as a scaffold of connectee.

The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL adapter also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI. When digested with BsmBI, this part can produce two sticky-ends. One can complement with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, while the other can complement with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`. With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), users can synthesize a 14-nucleotide-long Transactivator-like (TAL) protein to recogize their own connector and design their own polymerization. (Golden Gate Cloning) More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai. More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 612
Illegal AgeI site found at 724
Illegal AgeI site found at 1537
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1158

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1453000 short</partinfo>
@@ Line 7: / Line 6: @@
 The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000
-The TAL adapter part contains T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI, in order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), which is designed by iGEM12_Freiburg.
+In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL adapter also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
+When digested with BsmBI, this part can produce two sticky-ends. One can complement with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, while the other can complement with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.
+With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), users can synthesize a 14-nucleotide-long Transactivator-like (TAL) protein to recogize their own connector and design their own polymerization. (Golden Gate Cloning)
+More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai.
+More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.