Difference between revisions of "Part:BBa F2620"

Revision as of 18:03, 19 September 2006

A work in progress...

BBa_F2620

3OC₆HSL -> PoPS Receiver

tetR R0040	LuxR I0462	lux pR R0062

Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu] Last Update: Sept. 15th, 2006

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040. Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator.

Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics

Input Swing:	## nM 3OC₆HSL, exogenous
Output Swing:	#-# PoPS
Switch Point:	2 nM 3OC₆HSL, exogenous
L/H Latency:	# minutes

Key Subparts

BBa_R0040:	TetR-regulated operator
BBa_C0062:	luxR ORF
BBa_R0062:	LuxR-regulated operator

Transfer Function	Specificity
Latency	Stability

Demand

Transcription Demand:	## NTP per second ## polymerases sequestered
Translation Demand:	# charged tRNA per second # ribosomes sequestered

Stability

Genetic Stability:	##/## replication events (Lo Input/High Input)
Performance Stability:	##/## replication events (Lo Input/High Input)

Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC₆HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part, BBa_F2620.BBa_E0240. The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.

@@ Line 99: / Line 99: @@
 Crosstalk with systems containing ''TetR'' (''<partinfo>BBa_C0040</partinfo>'')<br>
 '''Characterization Conditions'''<br>
-All characterization was performed on ''<partinfo>pSB3k3</partinfo>'' using ''MG1655'' as the characterization chassis.  Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C.  Full characterization protocols can be found here.
+Device output was measured using the composite part, ''<partinfo>BBa_F2620</partinfo>.<partinfo>BBa_E0240</partinfo>''.  The characterization platform was BioBrick plasmid''<partinfo>pSB3k3</partinfo>'' with ''E. coli'' ''MG1655'' as chassis.  Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C.  Full characterization protocols can be found here.
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