Difference between revisions of "Part:BBa K1319002"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1319002 short</partinfo> | <partinfo>BBa_K1319002 short</partinfo> | ||
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* Y145W | * Y145W | ||
* H148R | * H148R | ||
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| + | ===References=== | ||
| + | * Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central PMCID: PMC1449651. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449651/?report=classic PubMed Central] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:00, 30 September 2014
RFC[25]-compatible dark quencher based on K1319000 (E0030)
This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).
Three mutations were introduced that eliminated fluorescence:
- L90I
- Y145W
- H148R
References
- Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central PMCID: PMC1449651. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449651/?report=classic PubMed Central]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]