Difference between revisions of "Part:BBa K1330000"
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<partinfo>BBa_K1330000 short</partinfo>
 
<partinfo>BBa_K1330000 short</partinfo>
  
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Spinach2 mutated without terminator
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This BioBrick contains the gene coding for the Spinach2.1 RNA, flanked by a tRNA scaffold sequence.
  
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===Usage and Biology===
 
===Usage and Biology===
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Spinach 2.1 RNA can bind and activate the fluorophore DFHBI, both in vivo and in vitro. RNA molecules can thus be fluorescently tagged, by gene fusion with this BioBrick.
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The flanking tRNA comes from human tRNALys3 and increases stability and folding efficiency of the RNA, leading to higher fluorescent signal.
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It is possible to detect signal from Spinach2.1 when expressed from moderate strength promoters, e.g. the [[Promoters/Catalog/Anderson|Anderson promoter library]].
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This part does not contain or need a Ribosome Binding Site.
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Revision as of 16:31, 22 October 2014

Spinach2.1 flanked by tRNALys3

This BioBrick contains the gene coding for the Spinach2.1 RNA, flanked by a tRNA scaffold sequence.

Usage and Biology

Spinach 2.1 RNA can bind and activate the fluorophore DFHBI, both in vivo and in vitro. RNA molecules can thus be fluorescently tagged, by gene fusion with this BioBrick. The flanking tRNA comes from human tRNALys3 and increases stability and folding efficiency of the RNA, leading to higher fluorescent signal. It is possible to detect signal from Spinach2.1 when expressed from moderate strength promoters, e.g. the Anderson promoter library.

This part does not contain or need a Ribosome Binding Site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]