Difference between revisions of "Part:BBa K1365006:Design"
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===Source=== | ===Source=== | ||
| − | Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain. | + | Obtained by PCR on the genome of ''Lactococcus lactis'' NZ9700, a nisin producing strain. |
===References=== | ===References=== | ||
Revision as of 02:34, 18 October 2014
NisR and NisK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATAAAATTTTAATAGT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA-3'
to PCR NisR and NisK from the genome and add the BioBrick prefix and suffix.
Changed the GTG start codon of NisR into a ATG.
Source
Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.