Difference between revisions of "Part:BBa K1439000:Design"
(→Source) |
(→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | OriTRP4 region contains some promoters,we put it in the back of terminator.In order to reduce the impact on protein expression. | + | OriTRP4 region contains some promoters, we put it in the back of terminator.In order to reduce the impact on protein expression. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 01:48, 30 September 2014
Origin of transfer for the RP4-plasmid nic region.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 20
Illegal NgoMIV site found at 30 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
OriTRP4 region contains some promoters, we put it in the back of terminator.In order to reduce the impact on protein expression.
Source
This part comes form the RP4 plasmid, from 51098bp to 51448bp.