Difference between revisions of "Part:BBa K1439000:Experience"

Revision as of 02:04, 30 September 2014

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Applications of BBa_K1439000

We have test the oriT BBa_K1439000 in a conjugation system in which pSB1C3 with help of RP4, and E. coli HB101 as donor and E.coil Top10 as recipient cells. We test the conjugative competence of oriT BBa_K1439000 by conjugate between HB101 and Top10. We use the RFP (BBa_J04450) marks the plasmids containing oriT BBa_K1439000. We get HB101 without streptomycin resistance form Zhigang Qiu. Top 10 has streptomycin resistance. After conjugation, we select recipient cells by streptomycin resistance LB medium and statistical number of red bacterial colony.

User Reviews

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 ===Applications of BBa_K1439000===
-We have test the oriT BBa_K1439000 in a conjugation system in which pSB1C3 with help of RP4, and E. coli HB101 as donor and E.coil Top10 as recipient cells. We test the conjugative competence of oriT BBa_K1439000 by conjugate between HB101 and Top10. We use the RFP (BBa_J04450) marks the plasmids containing oriT BBa_K1439000. We get HB101 with out streptomycin resistance form Zhigang Qiu. Top 10 has streptomycin resistance. After conjugation, we select recipient cells by streptomycin resistance LB medium and statistical number of red bacterial colony.
+We have test the oriT BBa_K1439000 in a conjugation system in which pSB1C3 with help of RP4, and E. coli HB101 as donor and E.coil Top10 as recipient cells. We test the conjugative competence of oriT BBa_K1439000 by conjugate between HB101 and Top10. We use the RFP (BBa_J04450) marks the plasmids containing oriT BBa_K1439000. We get HB101 without streptomycin resistance form Zhigang Qiu. Top 10 has streptomycin resistance. After conjugation, we select recipient cells by streptomycin resistance LB medium and statistical number of red bacterial colony.
 ===User Reviews===