Difference between revisions of "Part:BBa K1465201:Design"

(→‎Design Notes)
(→‎Source)
Line 19: Line 19:
 
===Source===
 
===Source===
  
−
Gensynthesis (gBlocks)
+
Gensynthesis ([http://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments gBlocks]) of the codon optimized sequence for ''Escherchia coli'' by using the [http://www.jcat.de/ Codon Adaptation Tool] and the [https://eu.idtdna.com/CodonOpt Codon Optimization from IDT]:
 +
 
 +
 
 +
BILD
  
 
===References===
 
===References===

Revision as of 21:58, 29 September 2014


Phosphoribulokinase prkA from Synechococcus elongatus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 657
    Illegal AgeI site found at 928
    Illegal AgeI site found at 999
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone using specific the Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_prkA pSB1C3_pre_prkA] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_prkA pSB1C3_suf_prkA]:
pSB1C3_pre_prkA (43 bp)

5'-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_prkA (43 bp)

5'-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the Phosphoribulokinase (prkA).

Source

Gensynthesis ([http://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments gBlocks]) of the codon optimized sequence for Escherchia coli by using the [http://www.jcat.de/ Codon Adaptation Tool] and the Codon Optimization from IDT:


BILD

References