Difference between revisions of "Part:BBa K1465201:Design"

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===Design Notes===
 
===Design Notes===
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<a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson-Assembly</a> using the Primer <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_prkA" target="_blank">pSB1C3_pre_prkA</a> and <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_prkA" target="_blank">pSB1C3_suf_prkA</a>
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*Amplification of the pSB1C3-Backbone using specific the Gibson-Primer <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_prkA" target="_blank">pSB1C3_pre_prkA</a> and <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_prkA" target="_blank">pSB1C3_suf_prkA</a>:<br>
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'''pSB1C3_pre_prkA (43 bp)'''<br>
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5'-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3'<br>
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'''pSB1C3_suf_prkA (43 bp)'''<br>
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5'-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3'<br>
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*<a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson-Assembly</a> of the pSB1C3-Backbone and the gBlock containing the sequence of the Phosphoribulokinase ('prkA').
  
 
===Source===
 
===Source===

Revision as of 21:23, 29 September 2014


Phosphoribulokinase prkA from Synechococcus elongatus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 657
    Illegal AgeI site found at 928
    Illegal AgeI site found at 999
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone using specific the Gibson-Primer <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_prkA" target="_blank">pSB1C3_pre_prkA</a> and <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_prkA" target="_blank">pSB1C3_suf_prkA</a>:
pSB1C3_pre_prkA (43 bp)

5'-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_prkA (43 bp)

5'-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3'
  • <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson-Assembly</a> of the pSB1C3-Backbone and the gBlock containing the sequence of the Phosphoribulokinase ('prkA').

Source

Gensynthesis (gBlocks)

References