Difference between revisions of "Part:BBa K1402002"

Latest revision as of 08:02, 2 October 2014

T7-RBS-MsbA-Terminator

ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. This ABC transporters MsbA can transport fatty acids out of the cell.The RBS and T7 promoter are in front of the MsbA coding sequence and T7 terminator is after MsbA. You can only use this part in E.coli BL21(DE3) or choose other parts we offer in other strains.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 10
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 61
Illegal XhoI site found at 1521
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 10
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 10
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1451

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 __NOTOC__
 <partinfo>BBa_K1402002 short</partinfo>
-ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. This ABC transporters MsbA can transport fatty acids out of the cell.The RBS and T7 promoter are in front of the MsbA coding sequence and T7 terminator is after MsbA. You can only use this part in E.coil BL21(DE3) or choose other parts we offer in other strains.
+ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. This ABC transporters MsbA can transport fatty acids out of the cell.The RBS and T7 promoter are in front of the MsbA coding sequence and T7 terminator is after MsbA. You can only use this part in E.coli BL21(DE3) or choose other parts we offer in other strains.
 <!-- Add more about the biology of this part here